Technical aspects of sperm DNA fragmentation testing, methods to select sperm with low DNA fragmentation, and usefulness of redox potential measurement in male infertility

Technical aspects of sperm DNA fragmentation testing, methods to select sperm with low DNA fragmentation, and usefulness of redox potential measurement in male infertility

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. We read with interest the thought provoking commentary by Dr. Henkel (1) in response to the practice recommendations by Agarwal et al. (2). Dr. Henkel appropriately pointed out the shortcomings of conventional semen analysis in predicting the outcome of assisted reproductive technologies (ART), to which we concur as d...

Clinical aspects of sperm DNA fragmentation detection and male infertility.

Over the past 25 years, various methods have been developed to measure sperm DNA strand breaks in situ. Currently, there are four major tests of sperm DNA fragmentation, including the Comet, Tunel, sperm chromatin structure assay (SCSA) and the acridine orange test (AOT). The Comet assay is a light microscope technique where the sperm cells are mixed with melted agarose and then placed on a gla...

Clinical usefulness of sperm DNA fragmentation testing

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. The search for the best biomarker for the diagnosis of male infertility is more evident in recent years as conventional semen analysis is not always an optimal predictor of male fertility. Since the advent of the intra-cytoplasmic sperm injection (ICSI) for male factor infertility, the pace of research related to sper...

Sperm DNA fragmentation testing

The editors of this book, Drs. Ashok Agarwal, Ahmad Majzoub, Chak-Lam Cho and Sandro Esteves, are to be congratulated for bringing together an extensive tome on the evaluation and management of sperm DNA fragmentation. The perspectives from North America, South America, Europe, Asia and the rest of the world provide an interesting snapshot into management of the unique phenomenon of sperm DNA d...

Implications of ejaculatory sperm DNA fragmentation on male infertility management